Most recently, LazB-F have been used to generate de novo thiopeptide pseudo-natural products with nanomolar affinity to Traf2- and NCK-interacting kinase (TNIK), an established target in several cancers.201,216 This was achieved by using a similar approach to previous mRNA display work209 but including an additional step in which counter-selection against immobilised TNIK enriches for binders to the targeted kinase. Here, TNIK is linked to cancer.