To further validate the expression changes of the differentially expressed immune checkpoint-related genes in clinical samples, and to enhance the reliability and accuracy of the research findings, we performed qPCR analysis of the expression differences of EGFR, TRIB3, CD4, and ZAP70 in 10 pairs of ccRCC tumor tissues and their adjacent normal tissues. This evidence concerns the gene CD4 and nonpapillary renal cell carcinoma.