DR5 receptors are commonly found overexpressed in cancer cells, such as melanoma cells, while 4‐1BB (CD137) is critical in CARs as it provides powerful costimulatory signals upon ligation.[41, 42, 43]  The CD3ζ signaling domain allows the CAR to mimic the natural T cell receptor (TCR) signaling and initiate T cell activation.[44] With our technology, we successfully generated CAR‐T cells with high transfection yield and potent cytotoxicity when co‐cultured with various melanoma cell lines. Here, TNFRSF9 is linked to melanoma.