Next, we validated the above finding with the publicly available Maris – 41 – FPKM – rsg001 RNA sequencing dataset of 39 human neuroblastoma cell lines (http://r2.amc.nl).[15] As shown in Figure 1D, PRKCQ‐AS1 was expressed at a significantly higher level in MYCN‐nonamplified than MYCN‐amplified human neuroblastoma cell lines. This evidence concerns the gene MYCN and neuroblastoma.