In vivo PHGDH expression was manipulated by injecting mice with GSCs expressing luciferase and DOX-induced PHGDH shRNA (Luc/DOX-shPHGDH), and DOX treatment upon tumor establishment drastically inhibited tumor growth (Supplementary Figure S3H, J) and extended lifespan (Supplementary Figure S3I, K). The gene discussed is PHGDH; the disease is neoplasm.