CD34 and neoplasm: To test anti-tumor proliferative capacity, we performed Hematoxylin and Eosin (H&E) staining, immunohistochemistry (IHC), and immunofluorescence (IF) staining on 4T1 tumor tissues after treatment to examine the cell apoptosis, proliferation marker (Ki67) and endothelial lining vascular marker (CD34).