PPARGC1A and neoplasm: While there was no difference in genome-wide promoter methylation, we observed hypermethylation of Cluster 1 (Figure 2E) gene promoters in AMPKα1/α2–deficient tumor-infiltrating Treg cells, as well as hypermethylation of Ppargc1a (PGC-1α) in tumor-infiltrating and splenic AMPKα1/α2–deficient Treg cells (Figure 6, A–C).