To test the relevance of our finding that Treg cells upregulate Prkaa2 in the TME (see Supplemental Figure 2B), we bred Treg cell–specific AMPKα1-deficient (Prkaa1fl/flFoxp3YFP–Cre) and AMPKα2-deficient (Prkaa2fl/flFoxp3YFP–Cre) mice and evaluated their response to B16 melanoma tumor grafts. This evidence concerns the gene PRKAA2 and melanoma.