Cluster 2 represented DEGs downregulated in AMPKα1/α2–deficient tumor-infiltrating Treg cells at day 12 after engraftment and included Prkaa1, along with a variety of Treg cell lineage markers (Foxp3, Il2ra, Stat5b) and mediators of Treg cell suppressive function (Tgfb1, Cxcr4, Itgae, Ccr8); notably, Cxcr4 and Ccr8 are known to be necessary for Treg cell suppressive function in the TME (27, 28). Here, TGFB1 is linked to neoplasm.