To determine the contribution of each AMPKα subunit to the transcriptional signature of AMPKα1/2–deficient Treg cells, we also sorted tumor-infiltrating Treg cells from Prkaa1fl/flFoxp3YFP–Cre and Prkaa2fl/flFoxp3YFP–Cre mice for RNA-seq at day 12 after engraftment. Here, PRKAA1 is linked to neoplasm.