GSEA revealed a positive enrichment of genes associated with allograft rejection and IFN-γ signaling as well as a negative enrichment of genes associated with angiogenesis in tumor-infiltrating AMPKα1/α2–deficient Treg cells (Supplemental Figure 3, A–C), consistent with loss of Treg cell function in the TME. This evidence concerns the gene IFNG and neoplasm.