When assessing the status of markers of Treg cell suppressive function, we found that loss of AMPKα1 or AMPKα2 had opposing effects on tumor-infiltrating Treg cell PD-1 expression, with loss of AMPKα1 leading to lower levels and loss of AMPKα2 leading to higher levels (Supplemental Figure 4N). This evidence concerns the gene PRKAA1 and neoplasm.