Because Prkaa1/2fl/flFoxp3YFP–Cre mice had significantly lower tumor burden relative to control mice at day 15 after engraftment, we also sorted tumor-infiltrating Treg cells for RNA-seq profiling when tumor burden was comparable (day 12 after engraftment) and identified 427 DEGs (Figure 2I and Supplemental Data Set 4); k-means clustering yielded 2 clusters of genes. Here, PRKAA1 is linked to neoplasm.