Collectively, these data indicate that tumor-infiltrating AMPKα1/α2–deficient Treg cells have impaired suppressive function, as evidenced by lower tumor burden over time, higher intratumoral CD8-to-Treg cell ratios, and higher frequency of IFN-γ+CD8+ cells in Prkaa1/2fl/flFoxp3YFP–Cre mice relative to controls. Here, PRKAA1 is linked to neoplasm.