These data show nanosecond laser treatment alters the RPE transcriptome across numerous biological pathways and can be viewed in conjunction with previous work in the ApoEnull and Nrf2null models of AMD showing subthreshold laser to alter matrix remodeling (Mmp2, Mmp3) and inflammation (e.g., FasL, IL1b, C3) genes (11, 40, 51). Here, C3 is linked to age-related macular degeneration.