However, there are several shortcomings in this study: Firstly, although we found that SCU pretreatment could reduce the serum ET-1 levels, increase the serum NO levels, and decrease the myocardial infarction area in I/R rats, indicating that SCU pretreatment may be beneficial for vasodilation and reperfusion, more direct evidence, such as changes in coronary artery diameter and coronary blood flow, is needed for further validation. The gene discussed is EDN1; the disease is myocardial infarction.