To determine whether the blunted immune response in mice infected with DLD-deficient parasites is due to impaired parasite proliferation (resulting in lower antigen release) or DLD is immunodominance [12], we analyzed PEPCK-specific CD4+ T cell response (strongly induced after L. major infection) at different times after infection with WT and DLD deficient parasites by flow cytometry (Fig 7A). Here, CD4 is linked to infection.