To validate that IFITs were the main factors that suppressed the replication of LSDVΔ012, bovine IFIT1 or IFIT5 were transfected into BHK-21 cells followed by infection with LSDV-eGFP or LSDVΔ012, and viral titers were determined by plaque assay described above. The gene discussed is IFIT5; the disease is infection.