SCD and fibrosarcoma: Both short-term co-cultures had a small number of upregulated proteins; no statistically significant proteins in the case of Naegleria-bacterial co-culture and five proteins in the case of Naegleria-fibrosarcoma co-culture, with only one upregulated protein unique to this condition (fatty acid desaturase A0A6A5C958 in fibrosarcoma co-culture).