Objectives: This study explored the impact of a 12-week walking and barre exercise program on depression levels (CES-D), stress hormones (dopamine, epinephrine, norepinephrine, cortisol, serotonin), high-sensitivity C-reactive protein (hs-CRP), and immunoglobulins (IgA, IgG, IgM) in women aged 65 and above. Here, CD40LG is linked to depressive symptom measurement.