Therefore, this study aimed to investigate the effects of 12-week walking and barre exercises on Center for Epidemiologic Studies Depression (CES-D), stress hormone (dopamine, epinephrine, norepinephrine, cortisol, serotonin), hs-CRP, and Ig (IgA, IgG, IgM) levels in older women aged ≥65 years to let older adults recognize the importance and necessity of exercise in improving physical and mental health and to enhance the quality of life in senescence through the healthy living of the older individuals. This evidence concerns the gene CD40LG and depressive disorder.