MFC data from a cohort of 68 subjects (27 APL and 41 non-APL AML cases) was mapped using four physical parameters, i.e., forward scatter (FSC) area (FSC-A) and height (FSC-H), side scatter (SSC) area (SSC-A) and height (SSC-H), and 6 fluorescent parameters (CD15, CD33, CD34, HLA-DR, CD117, and CD45). Here, CD33 is linked to acute myeloid leukemia.