Due to the need of considerable cholesterol exposure to induce advanced atherosclerosis development, extended cholesterol diet-feeding is required, and thus, APOE∗3-Leiden.CETP mice should be relatively old at the time of siRNA injection (approximately 30 weeks) compared with the 5-week or 6-week-old mice used in our previous Vwf-silencing experiments [11]. This evidence concerns the gene VWF and atherosclerosis.