In conclusion, our study examined PBRM1, a gene associated with PD1 therapeutic sensitivity in colorectal cancer, by whole-exome sequencing and found that its deficiency induced CD8+ T cell and NK cell infiltration in the microenvironment by regulating chromosomal accessibility, activating the NF-κB signaling pathway, and vesicle secretion (Figure 8). The gene discussed is CD8A; the disease is colorectal cancer.