To determine whether pY105-PKM2 drives stemness in lung cancer cells, we genetically modified A549 and H1299 cells to stably overexpress either wild-type PKM2 (PKM2WT) or its non-phosphorylatable mutant, PKM2Y105F, where tyrosine at position 105 was replaced with phenylalanine. Here, PKM is linked to lung carcinoma.