We enriched the CSC population through sphere culture from A549 and H1299 tumor-bearing mice and verified the enrichment using western blotting and flow cytometry to detect stem cell transcription factors (NANOG, OCT4, SOX2) and CSC surface markers (CD44, CD133) (Figure 1A-C, Figure S1D-F). The gene discussed is POU5F1; the disease is neoplasm.