One 33-year-old patient diagnosed in 2019 with HCC with infiltrative lesion in right lobe measuring 15.7 × 10.8 × 15.9 cm with vp3 PVTT in RPV and branches had serum PIVKA-II of 12335.2 mAU/mL and normal serum AFP before RT. This evidence concerns the gene AFP and hepatocellular carcinoma.