We further investigated conserved signaling pathways, CRH-1/CREB, AAK-2/AMPK, and JNK-1/JNK, results revealed that Berberine hydrochloride enhanced survival in crh-1(tz2), aak-2(ok524), and jnk-1(gk7) mutants after infection, similar to its effects in wild-type worms (Supplementary Figure S1A, D). Here, MAPK8 is linked to infection.