By contrast, since in mice Foxp3 is associated exclusively to Treg cells, Treg cell depletion (Foxp3-DTR mice) in para-orthopic NB murine model, resulted in 85% reduction of tumor volume and weight and increased amounts of cytokines (IFN-γ, TNF−α, IL-4, IL-6, and IL-10) in splenocytes as compared to control mice. The gene discussed is TNF; the disease is neoplasm.