Intestinal epithelial cells maintain the integrity and tight junction of intestinal epithelium by promoting the protein expression such as ZO-1 [26], oral gavage of mulberry anthocyanins improved intestinal barrier function and restored the expressions of intestinal tight junction protein (ZO-1 and CLDN3) in mice with dextran sulfate sodium-induced colitis [27]. Here, TJP1 is linked to colitis.