17 An example of the transformation of a 'cold' tumour to a 'hot' tumour is a genetically engineered trial of CF33-hNIS-antiPDL1, as the first human clinical trial (NCT05081492) of an OV combined with a checkpoint inhibitor to PD-L1 is currently being trialled as a treatment for metastatic breast cancer. 18,19. The gene discussed is CD274; the disease is neoplasm.