Finally, to validate the significance of high-order oligomerization of UXT in vivo, we injected RNA encoding GFP-tagged SOD1(A4V) into dorsal blastomeres, the precursors of neuronal cells, and monitored the motor neuron function of the subsequent tadpoles, utilizing this approach as an amyotrophic lateral sclerosis model.24 Here, SOD1 is linked to amyotrophic lateral sclerosis.