In response to d-flow under hypercholesterolemia, all MΦ clusters significantly accumulated, most notably MΦ3 and MΦ4 that highly expressed the markers of foam cells (Trem2, Lgals3, Gpnmb, Spp1, Lpl, and Fabp5) (Figure 4B, 4C Panels 9–10). The gene discussed is SPP1; the disease is familial hypercholesterolemia.