These data suggested that in BC, SETD8 may involve in regulating tumor growth through MYC, which was also ranked among the top candidates in our previous screening.[3] SETD8 has been previously reported to regulate gene expression by catalyzing H4K20me1.[13] To explore the impact of H4K20me1 on the MYC pathway, we performed a CUT&RUN assay using an anti‐H4K20me1 antibody in SETD8‐knockdown cell lines and observed reduced signals in the promoter and gene body regions of MYC target genes (Figure S2b, Supporting Information). Here, KMT5A is linked to breast cancer.