MAGEA3 and non-small cell lung carcinoma: To address this, we established a flow cytometry assay to measure specific HLA-peptide complexes expressed in NSCLC cells using the monoclonal antibody (mAb) 4F10 specific to HLA-A2 bound to the HER2 peptide KIFGSLAFL corresponding to amino acids 369–377 of the full-length HER2 protein (HER2369-377) [16] or the mAb 12B6 specific to HLA-A2 bound to the MAGE3 nonamer FLWGPRALV corresponding to amino acids 271–279 of the full-length MAGE3 protein (MAGE3271-279) [17] (Fig. 2A).