Additionally, Fusarium falciforme AUMC 16563’ pure asparaginase demonstrated anticancer activity against PC-3 (a prostate cell line) with an IC50 of 78.6 μg/mL, HePG-2 (a human hepatocellular carcinoma cell line) with an IC50 of 69.6 μg/mL, HCT-116 (a colon cell line) with an IC50 of 51.5 μg/mL and MCF-7 (a breast cancer cell line) with an IC50 of 32.8 μg/mL. This evidence concerns the gene ASPG and hepatocellular carcinoma.