This epitope is presented on the MHC class I molecule H-2Kb and is specifically recognized by T-cell receptor (TCR) transgenic OT-I CD8+ T cells (TCROT1).24 Then, we directly cocultured S100a1OE B16-OVA melanoma cells with TCROT1 to determine whether tumor-intrinsic S100A1 could directly affect T-cell function. The gene discussed is S100A1; the disease is melanoma.