This epitope is presented on the MHC class I molecule H-2Kb and is specifically recognized by T-cell receptor (TCR) transgenic OT-I CD8+ T cells (TCROT1).24 Then, we directly cocultured S100a1OE B16-OVA melanoma cells with TCROT1 to determine whether tumor-intrinsic S100A1 could directly affect T-cell function. This evidence concerns the gene CD8A and melanoma.