In addition, the tumor-infiltrating CD8+ T cells within the S100a1KD tumors demonstrated enhanced effector activity compared to those in the scramble control, as shown by their ability to secrete interferon-γ (IFN-γ), granzyme B (GZMB) and tumor necrosis factor-α (TNF-α) (Fig. 3d). This evidence concerns the gene TNF and neoplasm.