Immunofluorescence assays revealed that the co‐localization of perilipin, adipophilin, and TIP47 with F4/80 was significantly reduced in the atherosclerotic plaques of ApoE−/− mice injected with AAV‐circ‐PIAS1‐5 (Figure 4E), which indicates that circ‐PIAS1‐5 attenuated Hcy‐induced atherosclerosis by suppressing lipid accumulation in ApoE−/− mice. Here, PLIN1 is linked to atherosclerosis.