Several studies have analyzed in a direct obesity model by HFD, the expression of MAFbx, Murf1 proteins in the gastrocnemius muscle of male rodents (mice and Sprague-Dawley rats) obese by HFD finding an increase in the expression of these ubiquitin ligases [18, 19, 22, 23]. The gene discussed is TRIM63; the disease is obesity due to melanocortin 4 receptor deficiency.