Quantitative reverse transcription PCR (qRT-PCR) and WB assays showed that, compared to control groups, the expression of METTL3, ALKBH5, and FTO, but not METTL14 or WTAP, was significantly upregulated in FECD groups at both mRNA (Supplemental Fig. 7) and protein levels (Fig. 3A). Here, ALKBH5 is linked to Fuchs endothelial corneal dystrophy.