ACTA1 and Fuchs endothelial corneal dystrophy: WB assay and immunofluorescence (IF) staining confirmed the occurrence of cEndMT in FECD model, as showed by decreased expression of corneal endothelial markers (e.g., ZO-1, and Na+/K+-ATPase) and increased expression of mesenchymal markers (e.g., α-SMA and vimentin) (Fig. 4C, D; Supplemental Fig. 9A–D).