To specifically detect synapses formed by presynaptic PV interneuron and postsynaptic stroke-projecting neuron, we induced presynaptic GRASP in PV interneurons using the PV interneuron-specific enhancer, S5E249 (PV specificity: 91.3 ± 1.64%) and postsynaptic GRASP in the stroke-projecting neurons using retrograde AAV-Cre vector. The gene discussed is TAMALIN; the disease is Stroke.