We observed increased levels of pro-inflammatory factors (IL-1β, TNF-α, and IL-17) in the colon tissues of KOIEC mice compared to those in WT mice during DSS-induced colitis, via the enzyme-linked immunosorbent assay (ELISA) (Fig. S3C) and quantitative real-time PCR (qPCR) (Fig. S3D). This evidence concerns the gene TNF and colitis.