BNIP3 and osteosarcoma: However, in U2OS human osteosarcoma cells, TMEM11 deficiency lengthened cristae without affecting respiration or mtROS, likely because these cells mainly use glycolysis for energy.25,36 Recent findings also suggest a role for TMEM11 in inhibiting the BNIP3/BNIP3L-driven mitophagy pathway,25 although the loss of TMEM11 in T cells did not alter the number of mitochondria, indicating a possibly minor role in mitophagy, at least in Th1 cells.