RNA‐seq and RNA‐Bis‐seq analysis showed that RNF115 mRNA and its m5C modification level were elevated in HCC tumours, with the m5C modification site located in the 3′‐UTR, as confirmed by methylated RNA immunoprecipitation (MeRIP)‐quantitative polymerase chain reaction (qPCR; Figures 5A, B and S9A). The gene discussed is RNF115; the disease is neoplasm.