RNA‐seq and RNA‐Bis‐seq analysis showed that RNF115 mRNA and its m5C modification level were elevated in HCC tumours, with the m5C modification site located in the 3′‐UTR, as confirmed by methylated RNA immunoprecipitation (MeRIP)‐quantitative polymerase chain reaction (qPCR; Figures 5A, B and S9A). This evidence concerns the gene RNF115 and hepatocellular carcinoma.