Among the anthropometric, biochemical, and sex hormonal measurements analysed, NAFLD occurrence in PCOS was associated with specific thresholds: BMI > 26.8 kg/m2, AC > 88.3 cm, triglycerides > 1.57 mmol/L, TC > 4.67 mmol/L, LDL-C > 3.31 mmol/L, glucose > 4.83 mmol/L, insulin > 111.35 pmol/L, FT > 7.6 pg/mL and SHBG < 25 nmol/L. This evidence concerns the gene INS and polycystic ovary syndrome.