This validates the inCITE approach on the well-characterized microglial activation in AD, ALS and FTD, and extends previous work by showing that an NF-κB intermediate but TRAIL high transitional cluster exists in which the pathways activated are not canonical TNF/NF-κB response genes but more closely resemble the TRAIL signaling pathway. This evidence concerns the gene NFKB1 and amyotrophic lateral sclerosis.