Since high expression of P-gp mediates resistance of cancers to chemotherapeutic drugs, our findings of reduced P-gp and BCRP mRNA expression in hCLS1 knockdown hCMEC/D3 cells indicate that lowering CL levels in BBB endothelial cells could potentially be used as a therapeutic approach to enhance drug entry into the brain to treat brain tumors. Here, HCLS1 is linked to brain neoplasm.