To elucidate the mechanism underlying the protective effects of rIPC in AKI, we analyzed the therapeutic efficacy of rIPC in cisplatin-induced AKI models with NOX4tecko mice and TCMK-1 cells in which the NOX4 gene was silenced (siNOX4) or overexpressed by adenoviruses (Ad-NOX4). This evidence concerns the gene NOX4 and acute kidney injury.