Combined with the survival analysis of cell cyclin-related genes and their correlation analysis with IGF2BP3 expression in the TCGA-BLCA cohort and our clinical cohort, we found that CDK6 expression was positively correlated with IGF2BP3 expression in bladder cancer tissues and cell lines, and predicted poor prognosis in bladder cancer patients. This evidence concerns the gene IGF2BP3 and bladder transitional cell carcinoma.