Following moxibustion intervention, there was an enhancement in these indicators, indicating that moxibustion can enhance the expression levels of tight junction proteins Occludin and ZO-1 in the colonic mucosa of CIS model rats, reduce mucosal permeability, and improve intestinal mucosal barrier damage and gut dysbiosis, which aligns with relevant research results (25). The gene discussed is TJP1; the disease is in situ carcinoma.