In line with observations from the CD diet model, though no body weight difference was observed (Fig. 2R), the mice with IRF8 overexpression fed with HFD demonstrated similar metabolic damages, such as increased liver size (Fig. 2O), exacerbated hepatic steatosis (Fig. 2P), augmented liver weight (Fig. 2Q), higher levels of hepatic TG content (Fig. 2S), as well as higher plasma TG (Fig. 2T), AST (Fig. 2U), and ALT (Fig. 2V) levels. Here, GPT is linked to Hepatic steatosis.