CIB2 and deafness: Next, we performed NanoSPD assays in COS-7 cells using various mCherry-MYO10-CIB2 deafness-causing missense variants (p.Glu64Asp, p.Arg66Trp, p.Phe91Ser, p.Cys99Trp, p.Ile123Thr, p.Arg186Trp) and GFP-WHRN full-length constructs (Fig. 2B,C; Fig. S1).