In summary, we demonstrated that HMGB1 released from tumor cells treated with the recombinant adenoviruses ADVNE or ADVPPE binds to the TLR4 receptor on macrophages, activating the MyD88-NFκB-NLRP3 (ASC) pathway and inducing M1 macrophage polarization. The gene discussed is NLRP3; the disease is neoplasm.