Western blot analysis further revealed that the levels of cleaved Caspase-3 and N-GSDME in tumor cells were markedly elevated after infection with ADVNE or ADVPPE, whereas the HMGB1 levels decreased (Fig. 2H) and LDH release increased (Fig. 2I), all of which are indicators of pyroptosis [31]. This evidence concerns the gene CASP3 and neoplasm.