Novel mechanism of action can be established based on bioinformatics, in silico and in vitro work i.e. be like C. urens phytoconstituent Episesamin, targets the genes like CDK1, CDC25A, PLK1 which are majorly involved in cell cycle pathway and by the downregulating those genes, the cell cycle arrest may occur in metastasis and tumour growth of breast cancer cells (Fig. 6) [53]. The gene discussed is CDC25A; the disease is breast cancer.