Third, while the Wantai IgM anti-HEV enzyme-linked immunosorbent assay is widely accepted as being highly sensitive for diagnosing acute HEV infections, Huang et al observed that about 3% of acute viral hepatitis cases were negative for IgM anti-HEV but had a 4-fold rise in IgG anti-HEV in convalescent sera, indicating potential underestimation in our prevalence estimates due to the test's sensitivity [33]. Here, CD40LG is linked to animal viral hepatitis.