When the PDK4 variant was sequenced, of the DCM dogs 34/44 (77%) were wild-type (WT), 4/44 (9%) were heterozygous, and 6/44 (14%) were homozygous, and for the control dogs 23/30 (77%) were wild-type (WT), 4/30 (13%) were heterozygous, and 3/30 (10%) were homozygous. This evidence concerns the gene PDK4 and familial dilated cardiomyopathy.