This study conducted a gene functional enrichment analysis associated with invasion and metastasis in osteosarcoma cell lines, identifying hubs such as FGF2, ITGA6, ITGA7, ITGA8, ITGB3, ITGB4, COL3A1, COL4A1, COL4A2, COL6A2, COL6A6, IL6, CD44, CXCL8, and MMP‐2 as potential biomarkers due to their significance in osteosarcoma networks. This evidence concerns the gene COL4A2 and osteosarcoma.