In support, we detected significantly downregulated expression in the F. oxysporum of key genes involved in infection (SNF1, PL1, Fmk1, and Rho1), colonization (SIX8 and SIX1), pathogenicity (PelD), and hyphal development (FPD1) (Figure 4C and Supplementary Table S7). This evidence concerns the gene BSCL2 and infection.