Coordination issues could be spotted already at very early age (i.e., at P10) in Pfn2−/− mice by the characteristic hindlimb clasping when gently lifted by the tail instead of the normal hindlimb outward splaying (Supplementary Figure 3A), a phenotype typically present in mouse models with motor coordination and/or ataxia issues and cerebellar dysfunction (Lalonde and Strazielle, 2011; Yerger et al., 2022). This evidence concerns the gene PFN2 and Ataxia.